Positive fungal quantitative PCR and Th17 cytokine detection in bronchoalveolar lavage fluids: Complementary biomarkers of hypersensitivity pneumonitis?

Affiliation auteurs!!!! Error affiliation !!!!
TitrePositive fungal quantitative PCR and Th17 cytokine detection in bronchoalveolar lavage fluids: Complementary biomarkers of hypersensitivity pneumonitis?
Type de publicationJournal Article
Year of Publication2016
AuteursBellanger A-P, Gbaguidi-Haore H, Gondoin A, Pallandre J-R, Vacheyrou M, Valot B, Soumagne T, Reboux G, Dalphin J-C, Millon L
JournalJOURNAL OF IMMUNOLOGICAL METHODS
Volume434
Pagination61-65
Date PublishedJUL
Type of ArticleArticle
ISSN0022-1759
Mots-clésBronchoalveolar fluid, Eurotium amstelodami, Fungal QPCR, Hypersensitivity pneumonitis, IL-17, IL-23, Interstitial lung disease, Wallemia sebi
Résumé

Background: Interstitial lung disease (ILD) is a large group of diseases, including hypersensitivity pneumonitis (HP), idiopathic pulmonary fibrosis (IPF) and sarcoidosis. Objective: In this study, we aimed to identify bronchoalveolar lavage fluid (BALF) biomarkers which could be contributive for HP diagnosis. Methods: We analyzed 39 BALF samples from 12 ILD patients with sarcoidosis, 11 with IPF and 16 with HP. We determined the levels of 10 cytokines and carried out quantitative PCR for 10 microorganisms involved in farm-associated or domestic forms of HP. Results: IL-8, IL-6, TNF alpha, IL-17 and IL-23 levels were significantly higher in BALF samples from HP patients (p < 0.05, one-way Kruskal-Wallis analysis). QPCR tests for Eurotium amstelodami and Wallemia sebi were positively significantly more frequently for HP patients (p < 0.05, one-way Kruskal-Wallis). Conclusion: The biomarkers identified here can be detected in BALF, which could be routinely obtained as complementary analysis in ILD diagnosis. (C) 2016 Elsevier B.V. All rights reserved.
DOI10.1016/j.jim.2016.04.008