Single-Spore Extraction for Genetic Analyses of Arbuscular Mycorrhizal Fungi
| Affiliation auteurs | Affiliation ok |
| Titre | Single-Spore Extraction for Genetic Analyses of Arbuscular Mycorrhizal Fungi |
| Type de publication | Book Chapter |
| Year of Publication | 2020 |
| Auteurs | Redecker D |
| Editor | Ferrol N, Lanfranco L |
| Book Title | ARBUSCULAR MYCORRHIZAL FUNGI: Methods and Protocols |
| Series Title | Methods in Molecular Biology |
| Volume | 2146 |
| Pagination | 93-97 |
| Publisher | HUMANA PRESS INC |
| City | 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA |
| ISBN Number | 978-1-0716-0603-2; 978-1-0716-0602-5 |
| ISBN | 1064-3745 |
| Mots-clés | genotyping, Glomeromycota, Single-spore extraction |
| Résumé | Biomass of arbuscular mycorrhizal fungi (AMF, Glomeromycota) is often only available in small quantities as these fungi are obligate biotrophs and many species are difficult to cultivate under controlled conditions. Here, I describe a simple, efficient approach to produce crude extracts from single or a small number of spores that can be used for genotyping AMF. |
| DOI | 10.1007/978-1-0716-0603-2_8 |