Targeting Induced Local Lesions IN Genomes (TILLING) in Medicago truncatula
| Affiliation auteurs | !!!! Error affiliation !!!! |
| Titre | Targeting Induced Local Lesions IN Genomes (TILLING) in Medicago truncatula |
| Type de publication | Book Chapter |
| Year of Publication | 2018 |
| Auteurs | Sanchez M, Le Signor C, Aubert G, Darchy B, Gallardo K, Thompson RD |
| Editor | Canas LA, Beltran JP |
| Book Title | FUNCTIONAL GENOMICS IN MEDICAGO TRUNCATULA: METHODS AND PROTOCOLS |
| Series Title | Methods in Molecular Biology |
| Volume | 1822 |
| Pagination | 71-82 |
| Publisher | HUMANA PRESS INC |
| City | 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA |
| ISBN Number | 978-1-4939-8633-0; 978-1-4939-8632-3 |
| ISBN | 1064-3745 |
| Mots-clés | EMS chemical mutagenesis, Medicago truncatula, Reverse genetics, TILLING |
| Résumé | TILLING is a reverse genetics strategy that combines the high density of point mutations provided by traditional chemical mutagenesis with rapid screening of DNA pools from a mutagenized population for induced mutations (McCallum et al., Nat Biotechnol 18: 455-457, 2000). This high-throughput technique allows the identification of point mutations in any gene of interest. |
| DOI | 10.1007/978-1-4939-8633-0_5 |