Design of a degenerate primer pair to target a bacterial functional community: The hppd bacterial gene coding for the enzyme targeted by herbicides, a study case

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TitreDesign of a degenerate primer pair to target a bacterial functional community: The hppd bacterial gene coding for the enzyme targeted by herbicides, a study case
Type de publicationJournal Article
Year of Publication2020
AuteursThiour-Mauprivez C., Devers-Lamrani M., Mounier A., Beguet J., Spor A., Calvayrac C., Barthelmebs L., Martin-Laurent F.
JournalJOURNAL OF MICROBIOLOGICAL METHODS
Volume170
Pagination105839
Date PublishedMAR
Type of ArticleArticle
ISSN0167-7012
Mots-clésDegenerate primers, Hppd, Soil bacterial community
Résumé

The present work aimed to design a degenerate primer pair to target a large part of the hppd soil bacterial community, possibly affected by herbicides. We validated these primers by qPCR and high-throughput sequencing analysis of soil samples.

DOI10.1016/j.mimet.2020.105839