Odor-Induced Electrical and Calcium Signals from Olfactory Sensory Neurons In Situ
| Affiliation auteurs | Affiliation ok |
| Titre | Odor-Induced Electrical and Calcium Signals from Olfactory Sensory Neurons In Situ |
| Type de publication | Book Chapter |
| Year of Publication | 2018 |
| Auteurs | Grosmaitre X, Ma M |
| Editor | DeSouza FMS, Antunes G |
| Book Title | OLFACTORY RECEPTORS: METHODS AND PROTOCOLS |
| Series Title | Methods in Molecular Biology |
| Volume | 1820 |
| Pagination | 147-155 |
| Publisher | HUMANA PRESS INC |
| City | 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA |
| ISBN Number | 978-1-4939-8609-5; 978-1-4939-8608-8 |
| ISBN | 1064-3745 |
| Mots-clés | Calcium imaging, electrophysiology, GCaMP6, gene targeting, olfactory sensory neurons, Patch-clamp, transduction |
| Résumé | Electrophysiological recording and optical imaging enable the characterization of membrane and odorant response properties of olfactory sensory neurons (OSNs) in the nasal neuroepithelium. Here we describe a method to record the responses of mammalian OSNs to odorant stimulations in an ex vivo preparation of intact olfactory epithelium. The responses of individual OSNs with defined odorant receptor types can be monitored via patch-clamp recording or calcium imaging. |
| DOI | 10.1007/978-1-4939-8609-5_12 |