Kupffer Cell and Monocyte-Derived Macrophage Identification by Immunofluorescence on Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse Liver Sections

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TitreKupffer Cell and Monocyte-Derived Macrophage Identification by Immunofluorescence on Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse Liver Sections
Type de publicationBook Chapter
Year of Publication2020
AuteursGuillot A, Buch C, Jourdan T
EditorAouadi M, Azzimato V
Book TitleKUPFFER CELLS: Methods and Protocols
Series TitleMethods in Molecular Biology
Volume2164
Pagination45-53
PublisherHUMANA PRESS INC
City999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA
ISBN Number978-1-0716-0704-6; 978-1-0716-0703-9
ISBN1064-3745
Mots-clésCLEC4F/CLECSF13, Confocal microscopy, FFPE liver sections, IBA1, Immunofluorescence, Kupffer cell, Liver, macrophage, monocyte
Résumé

Kupffer cells are the liver-resident macrophages and represent the first line of defense between the pathogens circulating from the intestines through the portal vein and systemic circulation. Recent works have highlighted the complex heterogeneity of macrophage functions and origins, thus raising awareness on the need for a better characterization of macrophage populations. The immunohistochemistry method here described, allows for a rapid distinction between Kupffer cells and monocyte-derived macrophages present on formalin-fixed, paraffin-embedded mouse liver samples. This protocol has been optimized for its reproducibility, reliability, and simplicity.

DOI10.1007/978-1-0716-0704-6_6