Characterization of Carbapenem-Resistant Enterobacteriaceae Clinical Isolates in Al Thawra University Hospital, Sana'a, Yemen

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TitreCharacterization of Carbapenem-Resistant Enterobacteriaceae Clinical Isolates in Al Thawra University Hospital, Sana'a, Yemen
Type de publicationJournal Article
Year of Publication2020
AuteursAlsharapy SAhmed, Gharout-Sait A, Muggeo A, Guillard T, Cholley P, Brasme L, Bertrand X, Moghram GSaeed, Touati A, de Champs C
JournalMICROBIAL DRUG RESISTANCE
Volume26
Pagination211-217
Date PublishedMAR 1
Type of ArticleArticle
ISSN1076-6294
Mots-cléscarbapenemases, Enterobacteriaceae, NDM-1-like, OXA-48-like, Yemen
Résumé

{Objective: The aim of this study was to investigate the resistance mechanisms of carbapenem-resistant Enterobacteriaceae clinical strains recovered from Al Thawra University Hospital, Sana'a, Yemen. Methods: A total of 27 isolates showing decreased susceptibility to carbapenems were obtained from different clinical specimens in Al Thawra Hospital, Sana'a, Yemen. Strains were identified by Matrix Assisted Laser Desorption Ionization Time-Of-Flight spectroscopy. Susceptibility to antibiotics was determined by the disk diffusion method on Mueller Hinton agar. Carbapenemases-encoding genes, extended-spectrum beta-lactamases (ESBLs), and plasmid-mediated quinolone resistance (PMQR) genes were screened by PCR. Bacterial isolates were typed by multilocus sequence typing (MLST). Results: Carbapenemase genes detection and sequencing showed that 18 (66.7%) isolates were Klebsiella pneumoniae (NDM-1
DOI10.1089/mdr.2018.0443