Membrane Protein Production in Lactococcus lactis for Functional Studies
| Affiliation auteurs | !!!! Error affiliation !!!! |
| Titre | Membrane Protein Production in Lactococcus lactis for Functional Studies |
| Type de publication | Book Chapter |
| Year of Publication | 2016 |
| Auteurs | Seigneurin-Berny D, King MS, Sautron E, Moyet L, Catty P, Andre F, Rolland N, Kunji ERS, Frelet-Barrand A |
| Editor | MusVeteau I |
| Book Title | HETEROLOGOUS EXPRESSION OF MEMBRANE PROTEINS: METHODS AND PROTOCOLS, 2ND EDITION |
| Series Title | Methods in Molecular Biology |
| Volume | 1432 |
| Pagination | 79-101 |
| Publisher | HUMANA PRESS INC |
| City | 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA |
| ISBN Number | 978-1-4939-3637-3; 978-1-4939-3635-9 |
| ISBN | 1064-3745 |
| Mots-clés | Expression, Lactococcus lactis, Membrane proteins, Transport assays |
| Résumé | Due to their unique properties, expression and study of membrane proteins in heterologous systems remains difficult. Among the bacterial systems available, the Gram-positive lactic bacterium, Lactococcus lactis, traditionally used in food fermentations, is nowadays widely used for large-scale production and functional characterization of bacterial and eukaryotic membrane proteins. The aim of this chapter is to describe the different possibilities for the functional characterization of peripheral or intrinsic membrane proteins expressed in Lactococcus lactis |
| DOI | 10.1007/978-1-4939-3637-3_6 |