Characterization of eight microsatellite loci for the sea urchin Meoma ventricosa (Spatangoida, Brissidae) through Next Generation Sequencing

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TitreCharacterization of eight microsatellite loci for the sea urchin Meoma ventricosa (Spatangoida, Brissidae) through Next Generation Sequencing
Type de publicationJournal Article
Year of Publication2015
AuteursJossart Q, Geyer LB, Lessios H.A
JournalBIOCHEMICAL SYSTEMATICS AND ECOLOGY
Volume59
Pagination100-103
Date PublishedAPR
Type of ArticleArticle
ISSN0305-1978
Mots-clés454 method, Caribbean, echinoid, microsatellites, Tagged primer method
Résumé

Eight microsatellite loci were characterized for Meoma ventricosa (Lamarck, 1816), a burrowing sea urchin that can be afflicted by a bacterial disease causing localized mass mortality. For the analyzed population (29 individuals from St. Croix, US Virgin Islands), we observed 8.125 mean number of alleles, 0.640 mean observed heterozygosity (H-o) and 0.747 mean expected heterozygosity (H-e). Two loci showed significant deviations from Hardy-Weinberg equilibrium. Overall, the described loci were characterized by a moderately high level of polymorphism suggesting that these markers are useful for a population genetic study in the Caribbean Sea. (C) 2015 Elsevier Ltd. All. rights reserved.

DOI10.1016/j.bse.2015.01.013