Characterization of eight microsatellite loci for the sea urchin Meoma ventricosa (Spatangoida, Brissidae) through Next Generation Sequencing
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Titre | Characterization of eight microsatellite loci for the sea urchin Meoma ventricosa (Spatangoida, Brissidae) through Next Generation Sequencing |
Type de publication | Journal Article |
Year of Publication | 2015 |
Auteurs | Jossart Q, Geyer LB, Lessios H.A |
Journal | BIOCHEMICAL SYSTEMATICS AND ECOLOGY |
Volume | 59 |
Pagination | 100-103 |
Date Published | APR |
Type of Article | Article |
ISSN | 0305-1978 |
Mots-clés | 454 method, Caribbean, echinoid, microsatellites, Tagged primer method |
Résumé | Eight microsatellite loci were characterized for Meoma ventricosa (Lamarck, 1816), a burrowing sea urchin that can be afflicted by a bacterial disease causing localized mass mortality. For the analyzed population (29 individuals from St. Croix, US Virgin Islands), we observed 8.125 mean number of alleles, 0.640 mean observed heterozygosity (H-o) and 0.747 mean expected heterozygosity (H-e). Two loci showed significant deviations from Hardy-Weinberg equilibrium. Overall, the described loci were characterized by a moderately high level of polymorphism suggesting that these markers are useful for a population genetic study in the Caribbean Sea. (C) 2015 Elsevier Ltd. All. rights reserved. |
DOI | 10.1016/j.bse.2015.01.013 |