A Robust Generic Method for Grid Detection in White Light Microscopy Malassez Blade Images in the Context of Cell Counting
| Affiliation auteurs | !!!! Error affiliation !!!! |
| Titre | A Robust Generic Method for Grid Detection in White Light Microscopy Malassez Blade Images in the Context of Cell Counting |
| Type de publication | Journal Article |
| Year of Publication | 2015 |
| Auteurs | Marin A, Denimal E, Guyot S, Journaux L, Molin P |
| Journal | MICROSCOPY AND MICROANALYSIS |
| Volume | 21 |
| Pagination | 239-248 |
| Date Published | FEB |
| Type of Article | Article; Proceedings Paper |
| ISSN | 1431-9276 |
| Mots-clés | cell segmentation, Fourier transform, Hough transform, Image analysis, Malassez, morphological mathematics |
| Résumé | In biology, cell counting is a primary measurement and it is usually performed manually using hemocytometers such as Malassez blades. This work is tedious and can be automated using image processing. An algorithm based on Fourier transform filtering and the Hough transform was developed for Malassez blade grid extraction. This facilitates cell segmentation and counting within the grid. For the present work, a set of 137 images with high variability was processed. Grids were accurately detected in 98% of these images. |
| DOI | 10.1017/S1431927614013671 |