Electrochemical detection of the 2-isobuty1-3-methoxypyrazine model odorant based on odorant-binding proteins: The proof of concept
Affiliation auteurs | !!!! Error affiliation !!!! |
Titre | Electrochemical detection of the 2-isobuty1-3-methoxypyrazine model odorant based on odorant-binding proteins: The proof of concept |
Type de publication | Journal Article |
Year of Publication | 2015 |
Auteurs | Barou E, Sigoillot M, Bouvet M, Briand L, Meunier-Prest R |
Journal | BIOELECTROCHEMISTRY |
Volume | 101 |
Pagination | 28-34 |
Date Published | FEB |
Type of Article | Article |
ISSN | 1567-5394 |
Mots-clés | Electroanalysis, Electrochemistry, Fluorescence, Microcalorimetry, olfaction |
Résumé | {We developed an electrochemical assay for the detection of odorant molecules based on a rat odorant-binding protein (rOBP3). We demonstrated that rOBP3 cavity binds 2-methyl-1,4-naphtoquinone (MNQ), an electrochemical probe, as depicted from the decrease of its electrochemical signal, and deduced the dissociation constant |
DOI | 10.1016/j.bioelechem.2014.06.006 |