Size measuring techniques as tool to monitor pea proteins intramolecular crosslinking by transglutaminase treatment

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TitreSize measuring techniques as tool to monitor pea proteins intramolecular crosslinking by transglutaminase treatment
Type de publicationJournal Article
Year of Publication2016
AuteursDjoullah A, Krechiche G, Husson F, Saurel R
JournalFOOD CHEMISTRY
Volume190
Pagination197-200
Date PublishedJAN 1
Type of ArticleArticle
ISSN0308-8146
Mots-clésDynamic light scattering, Electrophoresis, Intramolecular crosslinks, Microbial transglutaminase, Pea albumin
Résumé

In this work, techniques for monitoring the intramolecular transglutaminase cross-links of pea proteins, based on protein size determination, were developed. Sodium dodecyl sulfate polyacrylamide gel electrophoresis profiles of transglutaminase-treated low concentration (0.01% w/w) pea albumin samples, compared to the untreated one (control), showed a higher electrophoretic migration of the major albumin fraction band (26 kDa), reflecting a decrease in protein size. This protein size decrease was confirmed, after DEAE column purification, by dynamic light scattering (DLS) where the hydrodynamic radius of treated samples appears to be reduced compared to the control one. (C) 2015 Elsevier Ltd. All rights reserved.

DOI10.1016/j.foodchem.2015.05.091