Size measuring techniques as tool to monitor pea proteins intramolecular crosslinking by transglutaminase treatment
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Titre | Size measuring techniques as tool to monitor pea proteins intramolecular crosslinking by transglutaminase treatment |
Type de publication | Journal Article |
Year of Publication | 2016 |
Auteurs | Djoullah A, Krechiche G, Husson F, Saurel R |
Journal | FOOD CHEMISTRY |
Volume | 190 |
Pagination | 197-200 |
Date Published | JAN 1 |
Type of Article | Article |
ISSN | 0308-8146 |
Mots-clés | Dynamic light scattering, Electrophoresis, Intramolecular crosslinks, Microbial transglutaminase, Pea albumin |
Résumé | In this work, techniques for monitoring the intramolecular transglutaminase cross-links of pea proteins, based on protein size determination, were developed. Sodium dodecyl sulfate polyacrylamide gel electrophoresis profiles of transglutaminase-treated low concentration (0.01% w/w) pea albumin samples, compared to the untreated one (control), showed a higher electrophoretic migration of the major albumin fraction band (26 kDa), reflecting a decrease in protein size. This protein size decrease was confirmed, after DEAE column purification, by dynamic light scattering (DLS) where the hydrodynamic radius of treated samples appears to be reduced compared to the control one. (C) 2015 Elsevier Ltd. All rights reserved. |
DOI | 10.1016/j.foodchem.2015.05.091 |