Swine cysticercosis in the Karangasem district of Bali, Indonesia: An evaluation of serological screening methods
| Affiliation auteurs | !!!! Error affiliation !!!! |
| Titre | Swine cysticercosis in the Karangasem district of Bali, Indonesia: An evaluation of serological screening methods |
| Type de publication | Journal Article |
| Year of Publication | 2016 |
| Auteurs | Swastika K, Dharmawan NSadra, I. Suardita K, I. Kepeng N, Wandra T, Sako Y, Okamoto M, Yanagida T, Sasaki M, Giraudoux P, Nakao M, Yoshida T, Diarthini LPutu Eka, I. Sudarmaja M, Purba IElisabeth, Budke CM, Ito A |
| Journal | ACTA TROPICA |
| Volume | 163 |
| Pagination | 46-53 |
| Date Published | NOV |
| Type of Article | Article |
| ISSN | 0001-706X |
| Mots-clés | Bali, Cysticercosis, ELISA, Indonesia, Necropsy, Pig, Serology, Taenia hydatigena, Taenia solium, Western blot |
| Résumé | {A serological assessment was undertaken on pigs from the Kubu and Abang sub-districts of Karangasem on the island of Bali, Indonesia, where earlier studies had detected patients with cysticercosis. Antigens purified from Taeniasolium cyst fluid by cation-exchange chromatography were used to evaluate antibody responses in the pigs and the serological tests were also evaluated using sera from pigs experimentally infected with T. solium eggs. A total of 392 serum samples from naturally exposed pigs were tested using an ELISA that could be read based on both a colour change perceptible by the naked eye and an ELISA based on absorbance values. Twenty six (6.6%) pigs were found seropositive by the naked-eye ELISA and were categorized into three groups: strongly positive (absorbance values >0.8 |
| DOI | 10.1016/j.actatropica.2016.07.022 |