Freezing and storage at -20 degrees C provides adequate preservation of Toxoplasma gondii DNA for retrospective molecular analysis
| Affiliation auteurs | !!!! Error affiliation !!!! |
| Titre | Freezing and storage at -20 degrees C provides adequate preservation of Toxoplasma gondii DNA for retrospective molecular analysis |
| Type de publication | Journal Article |
| Year of Publication | 2014 |
| Auteurs | Delhaes L, Filisetti D, Brenier-Pinchart M-P, Pelloux H, Yera H, Dalle F, Sterkers Y, Varlet-Marie E, Touafek F, Cassaing S, Bastien P |
| Journal | DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE |
| Volume | 80 |
| Pagination | 197-199 |
| Date Published | NOV |
| Type of Article | Article |
| ISSN | 0732-8893 |
| Mots-clés | Amniotic fluid, congenital toxoplasmosis, DNA storage, Real-Time PCR, Toxoplasma gondii |
| Résumé | Nucleic acid-based testing has become crucial for toxoplasmosis diagnosis. For retrospective (forensic or scientific) studies, optimal methods must be employed for DNA long-term storage. We compared Toxoplasma gondii detection before and after DNA storage using real-time PCR No significant differences were found depending on duration or storage conditions at -20 degrees C or -80 degrees C. (C) 2014 Elsevier Inc. All rights reserved. |
| DOI | 10.1016/j.diagmicrobio.2014.08.007 |