B cell depleting therapy regulates splenic and circulating T follicular helper cells in immune thrombocytopenia

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TitreB cell depleting therapy regulates splenic and circulating T follicular helper cells in immune thrombocytopenia
Type de publicationJournal Article
Year of Publication2017
AuteursAudia S, Rossato M, Trad M, Samson M, Santegoets K, Gautheron A, Bekker C, Facy O, Cheynel N, Ortega-Deballon P, Boulin M, Berthier S, Leguy-Seguin V, Martin L, Ciudad M, Janikashvili N, Saas P, Radstake T, Bonnotte B
JournalJOURNAL OF AUTOIMMUNITY
Volume77
Pagination89-95
Date PublishedFEB
Type of ArticleArticle
ISSN0896-8411
Mots-clésimmune thrombocytopenia, Rituximab, T follicular helper cells
Résumé

B cells are involved in immune thrombocytopenia (ITP) pathophysiology by producing antiplatelet auto antibodies. However more than a half of ITP patients do not respond to B cell depletion induced by rituximab (RTX). The persistence of splenic T follicular helper cells (TFH) that we demonstrated to be expanded during ITP and to support B cell differentiation and antiplatelet antibody-production may participate to RTX inefficiency. Whereas it is well established that the survival of TFH depends on B cells in animal models, nothing is known in humans yet. To determine the effect of B cell depletion on human TFH, we quantified B cells and TFH in the spleen and in the blood from ITP patients treated or not with RTX. We showed that B cell depletion led to a dramatic decrease in splenic TFH and in CXCL13 and IL-21, two cytokines predominantly produced by TFH. The absolute count of circulating TFH and serum CXCL13 also decreased after RTX treatment, whatever the therapeutic response. Therefore, we showed that the maintenance of TFH required B cells and that TFH are not involved in the inefficiency of RTX in ITP. (C) 2016 Elsevier Ltd. All rights reserved.

DOI10.1016/j.jaut.2016.11.002