Magnesium deficiency affects HNF1 beta expression in rat liver in vivo and in vitro

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TitreMagnesium deficiency affects HNF1 beta expression in rat liver in vivo and in vitro
Type de publicationJournal Article
Year of Publication2017
AuteursMartin H, Antoine D, Coste-Sarguet L, Sarr FSeydou, Richert L, Berthelot A
JournalMAGNESIUM RESEARCH
Volume30
Pagination98-105
Date PublishedJUL-SEP
Type of ArticleArticle
ISSN0953-1424
Mots-clésHNF1 beta, Magnesium, rat liver, vivo/vitro
Résumé

Hepatocyte nuclear factor 1 beta (HNF1 beta) is a transcription factor that is involved in embryonic development and tissue-specific gene expression in several organs, including the kidney and the liver. HNF1 beta mutations are associated with hypomagnesemia and renal magnesium wasting; however, to date, the exact molecular mechanism involved in this regulation is unclear. Furthermore, it is not known whether the Mg concentration could per se participate to this regulation by modifying HNF1 beta expression. We have studied in rats the effects of a 6-week diet with deficient or supplemented Mg concentrations compared to a diet with a standard Mg concentration on HNF1 beta protein expression. HNF1 beta expression was increased in the Mg-deficient group as compared to the other groups in the liver but not in the kidney. No changes in tissue Mg level were obtained in both organs. By contrast, a significant correlation between plasma Mg concentration and HNF1 beta level in the rat liver was evidenced. In rat hepatocyte cultures exposed for 72h to various extracellular Mg concentrations, HNF1 beta expression was modified after 72h of treatment of the hepatocytes with the lowest Mg concentrations as compared to the other Mg conditions. Moreover, these changes were correlated with extracellular but not intracellular Mg concentrations. In conclusion, HNF1 beta expression is modified by the extracellular Mg concentration in the liver, both in vivo and in vitro, suggesting regulations with membrane events in hepatocytes.

DOI10.1684/mrh.2017.0428