Steroidal saponins from Chlorophytum deistelianum

Phytochemical investigation of the aerial parts of Chlorophytum deistelianum led to the isolation of four previously undescribed steroidal saponins called chlorodeistelianosides A-D with five known ones. Their structures were established mainly by extensive 1D and 2D NMR spectroscopic techniques and mass spectrometry as (25R)-3 beta-[(beta-D-glucopyranosyl-(1 -> 3)-[alpha-L-rhamnopyranosyl-(1 -> 4)]-beta-D-xylopyranosyl-(1 -> 3)-[beta-D-glucopyranosyl-(1 -> 2)]-beta-D-glucopyranosyl-(1 -> 4)-beta-D-galactopyranosyl)-oxy]-5 alpha-spirostan-12-one, (24S,25S)-24-[(beta-D-glucopyranosyl)oxy]-3 beta-[(beta-D-glucopyranosyl-(1 -> 2)-[beta-D-xylopyranosyl-(1 -> 3)]-beta-D-glucopyranosyl-(1 -> 4)-beta-D-galactopyranosyl)oxy]-5 alpha-spirostan-12-one, (25R)-26-[(beta-D-glucopyranosyl)oxy]-2 alpha-hydroxy-22 alpha-methoxy-5 alpha-furostan-3 beta-yl beta-D-glucopyranosyl-(1 -> 2)-[beta-D-xylopyranosyl-(1 -> 3)]-beta-D-glucopyranosyl-(1 -> 4)-beta-D-galactopyranoside, and (25R)-26- [(beta-D-glucopyranosyl)oxy]-3 beta-[(beta-D-glucopyranosyl-(1 -> 2)-[beta-D-xylopyranosyl-(1 -> 3)]-beta-D-glucopyranosyl-(1 -> 4)-beta-D-galactopyranosyl)oxy]-5 alpha-furost-20(22)-en-12-one. Cytotoxicity of most compounds was evaluated against one human cancer cell line (SW480) and one rat cardiomyoblast cell line (H9c2). Among them, three known spirostane-type glycosides exhibited cytotoxicity on both cell lines with IC50 ranging from 8 to 10 mu M. (C) 2016 Elsevier Ltd. All rights reserved.